Human cytomegalovirus (HCMV) causes serious infections in immunocompromised patients and therapies to inhibit latent HCMV are not developed. Using CRISPR/Cas9, the authors were able to delete an important promoter region in HCMV.

Acquired immunodeficiency syndrome (AIDS) is a life-threatening condition caused by the human immunodeficiency virus (HIV). In this work, Takemaru et al explored the role of Coiled-Coil Domain-Containing 11 (CCDC11) in HIV-1 budding. Their results suggest that CCDC11 is critical for efficient HIV-1 budding, potentially indicating CCDC11 a viable target for antiviral therapeutics without major side effects.

Saccharomyces cerevisiae yeast is used to produce bioethanol, an alternative to fossil fuels. In this study, authors take advantage of this well studied yeast by genetically engineering them to increase fatty acid biosynthesis and culturing in a cost-effective wastewater based medium; potentially providing a sustainable alternative to petrochemicals.

The purpose of our study was to determine if direct administration of CXCL1/KC to cardiomyocytes causes negative changes to cell density or proliferation. This molecule has been shown to reduce inflammation in certain instances. Homocysteine models the direct effect of an inflammatory agent on cardiomyocytes. Our question was whether these molecules directly impact cell density through an interaction with the cell proliferation process. We hypothesized that cells treated with CXCL1/KC would maintain the same cell density as untreated cells. In contrast, cells treated with Homocysteine or both Homocysteine and CXCL1/KC, were expected to have a higher cell density that than that of untreated cells.

In this study, the authors investigated the biological mechanism underlying the actions of a traditional medicinal plant, Astragalus membranaceus. Using C. elegans as an experimental model, they tested the effects of AM root on heat stress responses. Their results suggest that AM root extract may enhance the activity of endogenous pathways that mediate cellular responses to heat stress.

Human amylase is important to digestion and has broad applications for therapeutic use in patients with pancreatic insufficiency. The authors present a method to increase amylase production in E. coli by adding the amino acids L-glutamate and L-glutamine.

Many species of trees are distributed widely around the world, though not always in a way that makes immediate sense. The authors here use genetic information to help explain the geographic distribution of various conifer species throughout the world.

Many diabetics agree that the current glucometer methods are invasive, inefficient, and unsustainable for measuring blood glucose. These authors investigate the possibility of using a non-invasive glucometer patch that predicts blood glucose from patient sweat, with high accuracy.

The Environmental Protection Agency (EPA) reports a significant number of heavy metal-contaminated sites across the United States. To address this public health concern, rhizoremediation using microbes has emerged as a promising solution. Here, a combination of soil microbes were inoculated in the rhizosphere in soil contaminated with 500 parts per million (ppm) of lead. Results showed rhizoremediation is an effective bioremediation strategy and may increase crop productivity by converting nonarable lands into arable lands.

The authors looked at 5-FU resistant HeLa cells and the ability of an herbal extract to show anti-inflammatory properties.